Table 2Single-cell RT-PCR analysis of the marker expression (ENK, SP and MOR mRNAs) among non-adapting and adapting MSNsa
ENK
SP
MOR
Non-adapting MSNs, % (n=59)
Adapting MSNs, % (n=33)
−
−
−
39 (23)
42 (14)
+
−
−
25 (15)
27 (9)
−
+
−
3 (2)
9 (3)
−
−
+
14 (8)
0 (0)
+
+
−
3 (2)
0 (0)
−
+
+
0 (0)
6 (2)
+
−
+
9 (5)
12 (4)
+
+
+
7 (4)
3 (1)
aPercentage of neurones displaying PCR amplicons for the different expression markers patterns relative to their spike frequency phenotype (non-adapting or adapting). No significant difference was found between both groups of MSNs considering each marker expression patterns, except for the exclusive expression of MOR (Fisher's exact test: P=0.05).